| | Club | Manager | League | Stadium | Location | Founded |
 | Biggleswade Town | | | Carlsberg Stadium | Biggleswade | 1874 |
| Stocksbridge Park Steels | | | Bracken Moor | Stocksbridge | 1986 |
| Witton Albion | Jon Macken | | Wincham Park | Northwich | 1887 |
| Taunton Town | | National League South | Cygnet Health Care Stadium | Taunton | 1947 |
| Leatherhead FC | | | Fetcham Grove | Leatherhead | 1907 |
| Tadcaster Albion | | | i2i Stadium | Tadcaster | 1892 |
| Daventry Town | | | Communications Park | Daventry | 1886 |
| Evesham United | | | Spiers and Hartwell Jubilee Stadium | Evesham | 1945 |
| Merstham FC | | | Moatside | Merstham | 1892 |
| Grays Athletic | | | Rookery Hill | Grays | 1890 |
| Bromsgrove Sporting | Tim Flowers | | Victoria Ground | Bromsgrove | 2009 |
| Winchester City | | | The City Ground | Winchester | 1891 |
| AFC Sudbury | | | King's Marsh | Sudbury | 1999 |
| Leiston | | | Victory Road | Leiston | 1880 |
| Concord Rangers | | | Thames Road | Canvey Island | 1967 |
| North Ferriby United | Steve Housham | | Grange Lane | North Ferriby | 1934 |
| Bury AFC | | | Stainton Park | Bury | 2019 |
| Frome Town | | | Blindmans Brewery Stadium | Frome | 1904 |
| Ramsbottom United | | | The Harry Williams Riverside Stadium | Ramsbottom | 1966 |
| Hythe Town | | | Reachfields Stadium | Hythe | 1992 |
| Kidsgrove Athletic | | | The Novus Stadium | Kidsgrove | 1952 |
| Burgess Hill Town | | | Leylands Park | Burgess Hill | 1882 |
| Sutton Coldfield Town | | | Central Ground | Sutton Coldfield | 1879 |
| Cinderford Town AFC | | | Causeway Ground | Cinderford | 1922 |
| Canvey Island | | | Park Lane | Canvey Island | 1926 |
| Stamford AFC | | | Zeeco Stadium | Stamford | 1896 |
| Ashington AFC | | | Woodhorn Lane | Ashington | 1883 |
| East Grinstead Town | | | East Court | East Grinstead | 1890 |
| Peterborough Sports | Luke Steele | National League North | Lincoln Road | Peterborough | 1908 |
| Chasetown FC | | | The Scholars Ground | Chasetown | 1954 |
| East Thurrock United | | | Rookery Hill | Corringham | 1969 |
| Bishop Auckland | | | Heritage Park | Bishop Auckland | 1886 |
| Congleton Town | | | Cheshire's Silk 106.9 Stadium | Congleton | 1901 |
| Newcastle Town | | | Lyme Valley Stadium | Newcastle-under-Lyme | 1964 |
| Needham Market | | | Bloomfields | Needham Market | 1919 |
| Cleethorpes Town | | | The Bradley Football Development Centre | Grimsby | 1998 |
| Bury Town | Cole Skuse | | Ram Meadow | Bury St Edmunds | 1872 |
| Harrow Borough | | | Earlsmead Stadium | Harrow | 1933 |
| Lowestoft Town | | | Crown Meadow | Lowestoft | 1887 |
| Walton Casuals | | | The Moatside Stadium | Walton-on-Thames | 1948 |
| Corinthian-Casuals | | | King George`s Field | Tolworth | 1939 |
| Nuneaton Borough | | | James Parnell Stadium | Nuneaton | 1889 |
| Vauxhall Motors | Alan Schumacker | | Rivacre Park | Ellesmere Port | 1963 |
| VCD Athletic | | | The Oakwood | Crayford | 1916 |
| Hitchin Town | | | Top Field | Hitchin | 1928 |
| Heybridge Swifts | Julian Dicks | | Scraley Road | Heybridge | 1880 |
| Wisbech Town | | | Fenland Stadium | Wisbech | 1920 |
| Highworth Town | | | The Elms Recreation Ground | Highworth | 1893 |
| Skelmersdale United | | | West Lancashire College Stadium | Skelmersdale | 1882 |
| Metropolitan Police | | | Imber Court | East Molesey | 1919 |
